General Cell Lines and Cell Cultures
Amsbio LLC MDA5/NF-kB Leeporter™ Luciferase Reporter-HEK293T Cell Line
MDA5/NF-kB Leeporter™ Luciferase Reporter-HEK293T Cell Line
ATCC Neisseria gonorrhoeae; genomic DNA
Genomic DNA isolated from Neisseria gonorrhoeae strain [ATCC 31426]. This bacterial strain is also available as ATCC Catalog No.: 53422™.
Eurofins Discoverx PathHunter CHRM1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0655C3)
The PathHunter CHRM1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen V2 Vasopressin Receptor Stable Cell Line, 2 vials. (HTS060C)
The ChemiScreen V2 Vasopressin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AVPR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation, 1 vial. (HTS057M)
The ChemiScreen PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PRLHR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen κ Opioid Receptor Membrane Preparation, 1 vial. (HTS095M)
The ChemiScreen κ Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRK1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter U2OS MC1R β-Arrestin Cell Line, 2 vials. (93-0861C3)
The PathHunter MC1R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC1R and EA-tagged β-Arrestin-2. Activation of MC1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Parotid, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Cell Applications Inc Proliferating Rat Brain Microvascular Endothelial Cells (RBMVEC), Adult
Proliferating Rat Brain Microvascular Endothelial Cells (RBMVEC), Adult
Eurofins Discoverx cAMP Hunter™ Human FPR3 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human FPR3 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure FPR3 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.